Efficient DNA double-strand break formation at single or multiple defined sites in the Saccharomyces cerevisiae genome.

ABSTRACT: DNA double-strand breaks (DSBs) are common genome lesions that threaten genome stability and cell survival. Cells use sophisticated repair machineries to detect and heal DSBs. To study DSB repair pathways and associated factors, inducible site-specific endonucleases have proven to be fundamental tools. In Saccharomyces cerevisiae, galactose-inducible rare-cutting endonucleases are commonly used to create a single DSB at a unique cleavage site. Galactose induction requires cell cultivation in suboptimal growth media, which is tedious especially when working with slow growing DSB repair mutants. Moreover, endonucleases that simultaneously create DSBs in multiple defined and unique loci of the yeast genome are not available, hindering studies of DSB repair in different genomic regions and chr

SUBMITTER: Gnugge R 

PROVIDER: S-EPMC7672422 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature