Development and characterization of a colon PDX model that reproduces drug responsiveness and the mutation profiles of its original tumor.

ABSTRACT: Cultures of primary tumors are very useful as a personalized screening system for effective therapeutic options. We here describe an effective method of reproducing human primary colon tumors through primary culture and a mouse xenograft model. A total of 199 primary colon tumor cultures were successfully established under optimized conditions to enrich for tumor cells and to expand it for long-term storage in liquid nitrogen. To examine whether these stored cultures retained original tumor properties, fifty primary cultures were xenografted into NOD-SCID mouse. Histological and tumor marker analysis of four representative tumor xenografts revealed that all of the xenograft retained its primary tumor characteristics. Oncomap analysis further showed no change in the major mutations in the x

SUBMITTER: Seol HS 

PROVIDER: S-EPMC7672533 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature