Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells.

ABSTRACT:

Background

Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate (MNPs@SiO₂(RITC)), even at 0.1 μg/μL. These protruded structures are involved in a cell's rigidity sensing, but how these NPs affect rigidity sensing is unknown.

Results

Here, we report that the rigidity sensing of human embryonic kidney (HEK293) cells was impaired even at 0.1 μg/μL of MNPs@SiO₂(RITC). At this concentration, cells were

SUBMITTER: Ketebo AA 

PROVIDER: S-EPMC7672867 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature