Clover: a clustering-oriented de novo assembler for Illumina sequences.

ABSTRACT:

Background

Next-generation sequencing technologies revolutionized genomics by producing high-throughput reads at low cost, and this progress has prompted the recent development of de novo assemblers. Multiple assembly methods based on de Bruijn graph have been shown to be efficient for Illumina reads. However, the sequencing errors generated by the sequencer complicate analysis of de novo assembly and influence the quality of downstream genomic researches.

Results

In this paper, we develop a de Bruijn assembler, called Clover (clustering-oriented de novo assembler), that utilizes a novel k-mer clustering approach from the overlap-layout-consensus concept to deal with the sequencing errors generated by the Illumina platform. We further evaluate Clover's performance against se

SUBMITTER: Hsieh MF 

PROVIDER: S-EPMC7672897 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature