Depletion of SNRNP200 inhibits the osteo-/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla.

ABSTRACT:

Background

Tissue regeneration mediated by mesenchymal stem cells (MSCs) is deemed a desirable way to repair teeth and craniomaxillofacial tissue defects. Nevertheless, the molecular mechanisms about cell proliferation and committed differentiation of MSCs remain obscure. Previous researches have proved that lysine demethylase 2A (KDM2A) performed significant function in the regulation of MSC proliferation and differentiation. SNRNP200, as a co-binding factor of KDM2A, its potential effect in regulating MSCs' function is still unclear. Therefore, stem cells from the apical papilla (SCAPs) were used to investigate the function of SNRNP200 in this research.

Methods

The alkaline phosphatase (ALP) activity assay, Alizarin Red staining, and osteogenesis-related gene expressions w

SUBMITTER: Su X 

PROVIDER: S-EPMC7672972 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature