Cytoplasm protein GFAP magnetic beads construction and application as cell separation target for brain tumors.

ABSTRACT:

Background

It is very important to develop a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function, for which the detection of circulating tumor cells (CTCs) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTCs separation target, a cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposome beads (IMLs). The validation and efficiency of this system in capturing CTCs for brain tumors were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed.

Results

Our data show that CTCs can be successfully isolated from CSF and blood samples from 32 children with brain tumor

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7673097 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature