Highly accurate long-read HiFi sequencing data for five complex genomes.

ABSTRACT: The PacBio^® HiFi sequencing method yields highly accurate long-read sequencing datasets with read lengths averaging 10-25 kb and accuracies greater than 99.5%. These accurate long reads can be used to improve results for complex applications such as single nucleotide and structural variant detection, genome assembly, assembly of difficult polyploid or highly repetitive genomes, and assembly of metagenomes. Currently, there is a need for sample data sets to both evaluate the benefits of these long accurate reads as well as for development of bioinformatic tools including genome assemblers, variant callers, and haplotyping algorithms. We present deep coverage HiFi datasets for five complex samples including the two inbred model genomes Mus musculus and Zea mays, as well as two com

SUBMITTER: Hon T 

PROVIDER: S-EPMC7673114 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature