Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle.

ABSTRACT: Regulating the thin-filament length in muscle is crucial for controlling the number of myosin motors that generate power. The giant protein nebulin forms a long slender filament that associates along the length of the thin filament in skeletal muscle with functions that remain largely obscure. Here nebulin's role in thin-filament length regulation was investigated by targeting entire super-repeats in the Neb gene; nebulin was either shortened or lengthened by 115 nm. Its effect on thin-filament length was studied using high-resolution structural and functional techniques. Results revealed that thin-filament length is strictly regulated by the length of nebulin in fast muscles. Nebulin's control is less tight in slow muscle types where a distal nebulin-free thin-filament segment exis

SUBMITTER: Kiss B 

PROVIDER: S-EPMC7673738 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature