CD94 Ex Vivo Cultures in a Bone Marrow Transplantation Setting.

ABSTRACT:

Background

Complementary, marrow donor-derived peripheral blood T-lymphocyte infusions enable consistent hematopoietic engraftment in lethally irradiated dog leukocyte antigen (DLA)-haploidentical littermate recipients, but at the cost of severe graft versus host disease (GVHD). Here, we explored whether CD94-selected and in vitro-expanded natural killer (NK) cells could be substituted for T-lymphocytes for enhancing marrow engraftment without causing severe GVHD.

Methods

Five dogs were conditioned with 700 cGy total body irradiation followed by infusion of DLA-haploidentical donor marrow and CD94-selected, in vitro-expanded NK cells. NK cells were infused at a median of 140 000 (range 78 000-317 000) cells/kg.

Results

Four dogs rejected their marrow grafts, whereas 1

SUBMITTER: Abrams K 

PROVIDER: S-EPMC7673772 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature