Trophic sympathetic influence weakens pro-contractile role of Cl^- channels in rat arteries during postnatal maturation.

ABSTRACT: Membrane transporters and their functional contribution in vasculature change during early postnatal development. Here we tested the hypothesis that the contribution of Cl^- channels to arterial contraction declines during early postnatal development and this decline is associated with the trophic sympathetic influence. Endothelium-denuded saphenous arteries from 1- to 2-week-old and 2- to 3-month-old male rats were used. Arterial contraction was assessed in the isometric myograph, in some experiments combined with measurements of membrane potential. mRNA and protein levels were determined by qPCR and Western blot. Sympathectomy was performed by treatment with guanethidine from the first postnatal day until 8-9-week age. Cl^- substitution in the solution as well as Cl

SUBMITTER: Kostyunina DS 

PROVIDER: S-EPMC7673994 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature