Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP.

ABSTRACT: Multidrug transporters can confer drug resistance on cells by extruding structurally unrelated compounds from the cellular interior. In transport assays, Hoechst 33342 (referred to as Hoechst) is a commonly used substrate, the fluorescence of which changes in the transport process. With three basic nitrogen atoms that can be protonated, Hoechst can exist as cationic and neutral species that have different fluorescence emissions and different abilities to diffuse across cell envelopes and interact with lipids and intracellular nucleic acids. Due to this complexity, the mechanism of Hoechst transport by multidrug transporters is poorly characterised. We investigated Hoechst transport by the bacterial major facilitator superfamily multidrug-proton antiporter LmrP in Lactococcus lactis and dev

SUBMITTER: Swain BM 

PROVIDER: S-EPMC7674423 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature