Comparative lipidomic analysis of mammalian retinal ganglion cells and Muller glia in situ and in vitro using High-Resolution Imaging Mass Spectrometry.

ABSTRACT: In order to better understand retinal physiology, alterations to which underlie some ocular diseases, we set out to establish the lipid signature of two fundamental cell types in the retina, Müller Glia and Retinal Ganglion Cells (RGCs). Moreover, we compared the lipid signature of these cells in sections (in situ), as well as after culturing the cells and isolating their cell membranes (in vitro). The lipidome of Müller glia and RGCs was analyzed in porcine retinal sections using Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS). Isolated membranes, as well as whole cells from primary cell cultures of RGCs and Müller glia, were printed onto glass slides using a non-contact microarrayer (Nano Plotter), and a LTQ-Orbitrap XL analyzer was used to scan the samp

SUBMITTER: Pereiro X 

PROVIDER: S-EPMC7674471 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature