The Genetic Architecture of Carbon Tetrachloride-Induced Liver Fibrosis in Mice.

ABSTRACT:

Background & aims

Liver fibrosis is a multifactorial trait that develops in response to chronic liver injury. Our aim was to characterize the genetic architecture of carbon tetrachloride (CCl₄)-induced liver fibrosis using the Hybrid Mouse Diversity Panel, a panel of more than 100 genetically distinct mouse strains optimized for genome-wide association studies and systems genetics.

Methods

Chronic liver injury was induced by CCl₄ injections twice weekly for 6 weeks. Four hundred thirty-seven mice received CCl₄ and 256 received vehicle, after which animals were euthanized for liver histology and gene expression. Using automated digital image analysis, we quantified fibrosis as the collagen proportionate area of the whole section, excluding nor

SUBMITTER: Tuominen I 

PROVIDER: S-EPMC7674618 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature