Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation.

ABSTRACT:

Objective

To explore the micromechanical, biochemical, and microstructural differences between bicuspid aortic valve aneurysm (BAV-A) and tricuspid aortic valve idiopathic degenerative aneurysm (DA), compared with normal aorta.

Methods

Aortic tissue was obtained from patients undergoing aneurysmal repair surgery (BAV-A; n = 15 and DA; n = 15). Control tissue was obtained from aortic punch biopsies during coronary artery bypass graft surgery (n = 9). Nanoindentation was used to determine the elastic modulus on the medial layer. Glycosaminoglycan, collagen, and elastin levels were measured using biochemical assays. Verhoeff Van Gieson-stained cross-sections were imaged for elastin microstructural quantification.

Results

The elastic modulus was more than 20% greater for

SUBMITTER: Chim YH 

PROVIDER: S-EPMC7674632 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature