Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor.

ABSTRACT: Cyclic adenosine monophosphate (cAMP) has been known to play an important role in regulating morphological development and antibiotic production in Streptomyces coelicolor. However, the functional connection between cAMP levels and antibiotic production and the mechanism by which cAMP regulates antibiotic production remain unclear. In this study, metabolomics- and transcriptomics-based multi-omics analysis was applied to S. coelicolor strains that either produce the secondary metabolite actinorhodin (Act) or lack most secondary metabolite biosynthesis pathways including Act. Comparative multi-omics analysis of the two strains revealed that intracellular and extracellular cAMP abundance was strongly correlated with actinorhodin production. Notably, supplementation of cAMP impr

SUBMITTER: Nitta K 

PROVIDER: S-EPMC7674942 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature