Multiparametric Profiling of Engineered Nanomaterials: Unmasking the Surface Coating Effect.

ABSTRACT: Despite considerable efforts, the properties that drive the cytotoxicity of engineered nanomaterials (ENMs) remain poorly understood. Here, the authors inverstigate a panel of 31 ENMs with different core chemistries and a variety of surface modifications using conventional in vitro assays coupled with omics-based approaches. Cytotoxicity screening and multiplex-based cytokine profiling reveals a good concordance between primary human monocyte-derived macrophages and the human monocyte-like cell line THP-1. Proteomics analysis following a low-dose exposure of cells suggests a nonspecific stress response to ENMs, while microarray-based profiling reveals significant changes in gene expression as a function of both surface modification and core chemistry. Pathway analysis highlights that the E

SUBMITTER: Gallud A 

PROVIDER: S-EPMC7675037 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature