Deletion of Histone Methyltransferase G9a Suppresses Mutant Kras-driven Pancreatic Carcinogenesis.

ABSTRACT:

Background/aim

The entire mechanisms by which epigenetic modifiers contribute to the development of pancreatic cancer remain unknown. Although the histone methyltransferase G9a is a promising target in human cancers, its role in pancreatic carcinogenesis has been under-studied. The aim of the study was to examine the role of G9a in pancreatic carcinogenesis by a gene-targeting mouse model.

Materials and methods

We established pancreas-specific G9a^flox/flox mice and crossed them with Ptf1a^Cre/; Kras^G12D/+ (KC) mice, which spontaneously develop pancreatic cancer. The phenotypes of the resulting KC mice with G9a deletion were examined. We analyzed transcriptomic data by microarray and genome-wide chromatin accessibility by transposase-accessible

SUBMITTER: Kato H 

PROVIDER: S-EPMC7675651 | biostudies-literature | 2020 Nov-Dec

REPOSITORIES: biostudies-literature