The long non-coding RNA HLNC1 potentiates hepatocellular carcinoma progression via interaction with USP49.

ABSTRACT:

Background

The hepatocellular carcinoma (HCC) represents a serious malignancy worldwide especially in China. Our transcriptome analysis identifies a novel long non-coding RNA (lncRNA) termed HLNC1. However, the function of HLNC1 in HCC remains to be determined.

Methods

Novel lncRNAs were screened using lncRNA profiling. Relative expression was quantified by qRT-PCR. In vitro experiments such as migration and viability assays were performed. In vivo implantation experiments were conducted to investigate tumorigenic functions. RNA-RNA interaction assay was performed to determine USP49 as HLNC1 binding partner.

Results

We found that HLNC1 was markedly upregulated in HCC samples and cell lines. HLNC1 could promote viability and migration of HCC cells. Meanwhile, we could

SUBMITTER: Qian X 

PROVIDER: S-EPMC7676201 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature