The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs).

ABSTRACT: Human adipose-derived stem cells (ASCs) have potential to improve wound healing; however, their equivalents from domestic animals have received less attention as an alternative cell-based therapy for animals or even humans. Hypoxia is essential for maintaining stem cell functionality in tissue-specific niches. However, a cellular response to low oxygen levels has not been demonstrated in pig ASCs. Hence, the goal of our study was to characterize ASCs isolated from the subcutaneous fat of domestic pigs (pASCs) and examine the effect of hypoxia on their proteome and functional characteristics that might reproduce pASCs wound healing ability. Analysis of immunophenotypic and functional markers demonstrated that pASCs exhibited characteristics of mesenchymal stem cells. Proteomic analysis reve

SUBMITTER: Bukowska J 

PROVIDER: S-EPMC7676232 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature