Characterization and Application of a Recombinant Exolytic GH50A β-Agarase from Cellvibrio sp. KY-GH-1 for Enzymatic Production of Neoagarobiose from Agarose.

ABSTRACT: Neoagarobiose (NA2) is the repeating disaccharide unit of agarose and possesses various promising biological activities. To identify an efficient exolytic β-agarase required for NA2 production from agarose, the GH50A β-agarase gene from agar-degrading Cellvibrio sp. KY-GH-1 was overexpressed as a recombinant His-tagged protein using the Escherichia coli expression system. GH50A β-agarase that consists of 797 amino acids was able to produce predominantly NA2 from agarose at an optimal temperature and pH of 35 °C and 7.5, respectively. The enzyme was stable up to 35 °C and within a pH range of 7.0-9.0. The K _m, V _max, K _cat, and K _cat/K _m values of the enzyme were 26.5 mg/mL, 16.9 U/mg, 25

SUBMITTER: Kwon M 

PROVIDER: S-EPMC7676358 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature