Multi-targeted gene silencing strategies inhibit replication of Canine morbillivirus.

ABSTRACT:

Background

Canine morbilivirus (canine distemper virus, CDV) is a highly contagious pathogen associated with high morbidity and mortality in susceptible carnivores. Although there are CDV vaccines available, the disease poses a huge threat to dogs and wildlife hosts due to vaccine failures and lack of effective treatment. Thus, the development of therapeutics is an urgent need to achieve rapid outbreak control and reduce mortality in target species. Gene silencing by RNA interference has emerged as a promising therapeutic approach against different human and animal viruses. In this study, plasmid-based short hairpin RNAs (shRNAs) against three different regions in either CDV nucleoprotein (N), or large polymerase (L) genes and recombinant adenovirus-expressing N-specific multi-shRN

SUBMITTER: de Carvalho OV 

PROVIDER: S-EPMC7676405 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature