A Custom Ultra-Low-Cost 3D Bioprinter Supports Cell Growth and Differentiation.

ABSTRACT: Advances in 3D bioprinting have allowed the use of stem cells along with biomaterials and growth factors toward novel tissue engineering approaches. However, the cost of these systems along with their consumables is currently extremely high, limiting their applicability. To address this, we converted a 3D printer into an open source 3D bioprinter and produced a customized bioink based on accessible alginate/gelatin precursors, leading to a cost-effective solution. The bioprinter's resolution, including line width, spreading ratio and extrusion uniformity measurements, along with the rheological properties of the bioinks were analyzed, revealing high bioprinting accuracy within the printability window. Following the bioprinting process, cell survival and proliferation were validated on HeLa

SUBMITTER: Ioannidis K 

PROVIDER: S-EPMC7676439 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature