Affinity-Restricted Memory B Cells Dominate Recall Responses to Heterologous Flaviviruses.

ABSTRACT: Memory B cells (MBCs) can respond to heterologous antigens either by molding new specificities through secondary germinal centers (GCs) or by selecting preexisting clones without further affinity maturation. To distinguish these mechanisms in flavivirus infections and immunizations, we studied recall responses to envelope protein domain III (DIII). Conditional deletion of activation-induced cytidine deaminase (AID) between heterologous challenges of West Nile, Japanese encephalitis, Zika, and dengue viruses did not affect recall responses. DIII-specific MBCs were contained mostly within the plasma-cell-biased CD80⁺ subset, and few GCs arose following heterologous boosters, demonstrating that recall responses are confined by preexisting clonal diversity. Measurement of monoclonal

SUBMITTER: Wong R 

PROVIDER: S-EPMC7677180 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature