Dye Transport through Bilayers Agrees with Lipid Electropore Molecular Dynamics.

ABSTRACT: Although transport of molecules into cells via electroporation is a common biomedical procedure, its protocols are often based on trial and error. Despite a long history of theoretical effort, the underlying mechanisms of cell membrane electroporation are not sufficiently elucidated, in part, because of the number of independent fitting parameters needed to link theory to experiment. Here, we ask if the electroporation behavior of a reduced cell membrane is consistent with time-resolved, atomistic, molecular dynamics (MD) simulations of phospholipid bilayers responding to electric fields. To avoid solvent and tension effects, giant unilamellar vesicles (GUVs) were used, and transport kinetics were measured by the entry of the impermeant fluorescent dye calcein. Because the timescale of ele

SUBMITTER: Sozer EB 

PROVIDER: S-EPMC7677249 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature