Balance of Na⁺, K⁺, and Cl^- Unidirectional Fluxes in Normal and Apoptotic U937 Cells Computed With All Main Types of Cotransporters.

ABSTRACT: Fluxes of monovalent ions through the multiple pathways of the plasma membrane are highly interdependent, and their assessment by direct measurement is difficult or even impossible. Computation of the entire flux balance helps to identify partial flows and study the functional expression of individual transporters. Our previous computation of unidirectional fluxes in real cells ignored the ubiquitous cotransporters NKCC and KCC. Here, we present an analysis of the entire balance of unidirectional Na⁺, K⁺, and Cl^- fluxes through the plasma membrane in human lymphoid U937 cells, taking into account not only the Na/K pump and electroconductive channels but all major types of cotransporters NC, NKCC, and KCC. Our calculations use flux equations based on the fun

SUBMITTER: Yurinskaya VE 

PROVIDER: S-EPMC7677585 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature