Pathway engineering of Escherichia coli for one-step fermentative production of L-theanine from sugars and ethylamine.

ABSTRACT: L-theanine is the most abundant free amino acid in tea that offers various favorable physiological and pharmacological effects. Bacterial enzyme of γ-glutamylmethylamide synthetase (GMAS) can catalyze the synthesis of theanine from glutamate, ethylamine and ATP, but the manufacturing cost is uncompetitive due to the expensive substrates and complex processes. In this study, we described pathway engineering of wild-type Escherichia coli for one-step fermentative production of theanine from sugars and ethylamine. First, the synthetic pathway of theanine was conducted by heterologous introduction of a novel GMAS from Paracoccus aminovorans. A xylose-induced T7 RNA polymerase-P _T7 promoter system was used to enhance and control gmas gene expression. Next,

SUBMITTER: Fan X 

PROVIDER: S-EPMC7677707 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature