Bivalent genes that undergo transcriptional switching identify networks of key regulators of embryonic stem cell differentiation.

ABSTRACT:

Background

Bivalent promoters marked with both H3K27me3 and H3K4me3 histone modifications are characteristic of poised promoters in embryonic stem (ES) cells. The model of poised promoters postulates that bivalent chromatin in ES cells is resolved to monovalency upon differntiation. With the availability of single-cell RNA sequencing (scRNA-seq) data, subsequent switches in transcriptional state at bivalent promoters can be studied more closely.

Results

We develop an approach for capturing genes undergoing transcriptional switching by detecting 'bimodal' gene expression patterns from scRNA-seq data. We integrate the identification of bimodal genes in ES cell differentiation with analysis of chromatin state, and identify clear cell-state dependent patterns of bimodal, bivalen

SUBMITTER: Jeon AJ 

PROVIDER: S-EPMC7677846 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature