Comparative analysis of the transcriptomes of EDL, psoas, and soleus muscles from mice.

ABSTRACT:

Background

Individual skeletal muscles have evolved to perform specific tasks based on their molecular composition. In general, muscle fibers are characterized as either fast-twitch or slow-twitch based on their myosin heavy chain isoform profiles. This approach made sense in the early days of muscle studies when SDS-PAGE was the primary tool for mapping fiber type. However, Next Generation Sequencing tools permit analysis of the entire muscle transcriptome in a single sample, which allows for more precise characterization of differences among fiber types, including distinguishing between different isoforms of specific proteins. We demonstrate the power of this approach by comparing the differential gene expression patterns of extensor digitorum longus (EDL), psoas, and soleus from

SUBMITTER: Hettige P 

PROVIDER: S-EPMC7678079 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature