High-fat diet-induced and genetically inherited obesity differentially alters DNA methylation profile in the germline of adult male rats.

ABSTRACT:

Background

Paternal obesity has been associated with reduced live birth rates. It could lead to inheritance of metabolic disturbances to the offspring through epigenetic mechanisms. However, obesity is a multifactorial disorder with genetic or environmental causes. Earlier we had demonstrated differential effects of high-fat diet-induced obesity (DIO) and genetically inherited obesity (GIO) on metabolic, hormonal profile, male fertility, and spermatogenesis using two rat models. The present study aimed to understand the effect of DIO and GIO on DNA methylation in male germline, and its subsequent effects on the resorbed (post-implantation embryo loss) and normal embryos. First, we assessed the DNA methylation enzymatic machinery in the testis by Real-Time PCR, followed global DNA m

SUBMITTER: Deshpande SS 

PROVIDER: S-EPMC7678167 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature