An in vitro bioengineered model of the human arterial neurovascular unit to study neurodegenerative diseases.

ABSTRACT:

Introduction

The neurovascular unit (NVU) - the interaction between the neurons and the cerebrovasculature - is increasingly important to interrogate through human-based experimental models. Although advanced models of cerebral capillaries have been developed in the last decade, there is currently no in vitro 3-dimensional (3D) perfusible model of the human cortical arterial NVU.

Method

We used a tissue-engineering technique to develop a scaffold-directed, perfusible, 3D human NVU that is cultured in native-like flow conditions that mimics the anatomy and physiology of cortical penetrating arteries.

Results

This system, composed of primary human vascular cells (endothelial cells, smooth muscle cells and astrocytes) and induced pluripotent stem cell (iPSC) derived neur

SUBMITTER: Robert J 

PROVIDER: S-EPMC7678181 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature