Urolithin A augments angiogenic pathways in skeletal muscle by bolstering NAD⁺ and SIRT1.

ABSTRACT: Urolithin A (UA) is a natural compound that is known to improve muscle function. In this work we sought to evaluate the effect of UA on muscle angiogenesis and identify the underlying molecular mechanisms. C57BL/6 mice were administered with UA (10 mg/body weight) for 12-16 weeks. ATP levels and NAD⁺ levels were measured using in vivo ³¹P NMR and HPLC, respectively. UA significantly increased ATP and NAD⁺ levels in mice skeletal muscle. Unbiased transcriptomics analysis followed by Ingenuity Pathway Analysis (IPA) revealed upregulation of angiogenic pathways upon UA supplementation in murine muscle. The expression of the differentially regulated genes were validated using quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (I

SUBMITTER: Ghosh N 

PROVIDER: S-EPMC7678835 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature