LRRC75A-AS1 targets miR-199b-5p/PDCD4 axis to repress multiple myeloma.

ABSTRACT:

Background

Multiple functions of miR-199b-5p in diseases have been demonstrated by existing studies. However, never has the correlation between miR-199b-5p and multiple myeloma (MM) been established.

Methods

qRT-PCR analyzed RNA expression and western blot measured protein expression. Cell proliferation ability was tested via colony formation and EdU assays, and apoptosis was determined via TUNEL, flow cytometry and detection of apoptosis-related proteins. Position of LRRC75A antisense RNA 1 (LRRC75A-AS1) was recognized by FISH assay. RIP, RNA pull-down and luciferase reporter experiments explored the molecular interplay.

Results

GEO (Gene Expression Omnibus) data revealed miR-199b-5p upregulation in MM specimens, and qRT-PCR data verified miR-199b-5p upregulation in

SUBMITTER: Pang Q 

PROVIDER: S-EPMC7678947 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature