In vivo Characterization of Four ¹⁸F-Labeled S1PR1 Tracers for Neuroinflammation.

ABSTRACT:

Purpose

The sphingosine-1-phosphate receptor 1 (S1PR1) is an important biomarker for imaging inflammation in the central nervous system (CNS). Herein, we report our recent evaluation of four ¹⁸F-labeled S1PR1 tracers (¹⁸F-TZ43113, ¹⁸F-TZ35104, ¹⁸F-TZ4877, and ¹⁸F-TZ4881) in a rat model of multiple sclerosis (MS).

Procedures

MicroPET studies of each tracer's uptake and kinetics were performed in an experimental autoimmune encephalomyelitis (EAE) rat model of MS to quantify upregulated S1PR1 expression in the lumbar spinal cord of EAE rats. Western blot analysis was conducted to confirm the differences in the expression of S1PR1 protein level between EAE and sham rats. Radiometabolite analysis was performed for the most pr

SUBMITTER: Liu H 

PROVIDER: S-EPMC7679043 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature