Integration of single-cell datasets reveals novel transcriptomic signatures of β-cells in human type 2 diabetes.

ABSTRACT: Pancreatic islet β-cell failure is key to the onset and progression of type 2 diabetes (T2D). The advent of single-cell RNA sequencing (scRNA-seq) has opened the possibility to determine transcriptional signatures specifically relevant for T2D at the β-cell level. Yet, applications of this technique have been underwhelming, as three independent studies failed to show shared differentially expressed genes in T2D β-cells. We performed an integrative analysis of the available datasets from these studies to overcome confounding sources of variability and better highlight common T2D β-cell transcriptomic signatures. After removing low-quality transcriptomes, we retained 3046 single cells expressing 27 931 genes. Cells were integrated to attenuate dataset-specific biases, and clustered into cell

SUBMITTER: Bosi E 

PROVIDER: S-EPMC7679065 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature