Consensus mutagenesis approach improves the thermal stability of system x_c ^- transporter, xCT, and enables cryo-EM analyses.

ABSTRACT: System x_c ^- is an amino acid antiporter that imports L-cystine into cells and exports intracellular L-glutamate, at a 1:1 ratio. As L-cystine is an essential precursor for glutathione synthesis, system x_c ^- supports tumor cell growth through glutathione-based oxidative stress resistance and is considered as a potential therapeutic target for cancer treatment. System x_c ^- consists of two subunits, the light chain subunit SLC7A11 (xCT) and the heavy chain subunit SLC3A2 (also known as CD98hc or 4F2hc), which are linked by a conserved disulfide bridge. Although the recent structures of another SLC7 member, L-type amino acid transporter 1 (LAT1) in complex with CD98hc, have provided the structural basis toward understanding the

SUBMITTER: Oda K 

PROVIDER: S-EPMC7679960 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature