STAT4 targets KISS1 to promote the apoptosis of ovarian granulosa cells.

ABSTRACT:

Background

In mammals, it is known that the estradiol-17β (E2) is mainly synthetized in ovarian granulosa cells (GCs), and the excessive apoptosis of GCs induces the follicular atresia. Many studies have implicated the essential role of KISS1, with the pro-synthetic effect of E2 and the anti-apoptotic effect on GCs, in the mammalian folliculogenesis, and several STAT4 potential binding sites were previously predicted on the promoter of KISS1 in pigs. However, the biological effects of STAT4 on GCs and the molecular regulation between STAT4 and KISS1 remained largely unknown.

Methods

Using the porcine GCs as the cellular model, the overexpression plasmid, small interfering RNA, 5'-deletion and luciferase assay were applied to investigate the molecular mechanisms for STAT4 reg

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC7679982 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature