Bioinformatics Analysis Identifies a Novel Role of GINS1 Gene in Colorectal Cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) is one of the most lethal malignancies and the incidence of CRC has been on the rise. Herein, we aimed to identify effective biomarkers for early diagnosis and treatment of colorectal cancer via bioinformatic tools.

Methods

To identify differentially expressed genes (DEGs) in CRC, we downloaded CRC gene expression data from GSE24514 and GSE110223 datasets in Gene Expression Omnibus (GEO) and employed R to analyze the data. We further performed functional enrichment analysis of the DEGs on the DAVID gene ontology analysis tool. STRING database and Cytoscape visualization tool were employed to construct a PPI (protein-protein interaction) network and establish intensive intervals in the network. Immunohistochemistry, qRT-PCR and Western blott

SUBMITTER: Bu F 

PROVIDER: S-EPMC7680165 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature