Tuft Cells Inhibit Pancreatic Tumorigenesis in Mice by Producing Prostaglandin D₂.

ABSTRACT:

Background & aims

Development of pancreatic ductal adenocarcinoma (PDA) involves acinar to ductal metaplasia and genesis of tuft cells. It has been a challenge to study these rare cells because of the lack of animal models. We investigated the role of tuft cells in pancreatic tumorigenesis.

Methods

We performed studies with LSL-Kras^G12D/+;Ptf1a^Cre/+ mice (KC; develop pancreatic tumors), KC mice crossed with mice with pancreatic disruption of Pou2f3 (KPouC mice; do not develop tuft cells), or mice with pancreatic disruption of the hematopoietic prostaglandin D synthase gene (Hpgds, KHC mice) and wild-type mice. Mice were allowed to age or were given caerulein to induce pancreatitis; pancreata were collected and analyzed by histology, immunohistochemistry

SUBMITTER: DelGiorno KE 

PROVIDER: S-EPMC7680354 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature