Epithelial-mesenchymal transition and metastatic ability of CD133⁺ colorectal cancer stem-like cells under hypoxia.

ABSTRACT: Although CD133 is a representative cancer stem cell marker, its function in tumor aggressiveness under hypoxia remains unclear. Therefore, the present study aimed to investigate the associations between CD133, the epithelial-mesenchymal transition and distant metastasis in colorectal cancer. CD133⁺ and CD133^- cells were isolated from a single colorectal cancer cell line LoVo, and their adhesive and migratory properties were compared under hypoxic conditions. Immunostaining analysis was performed to determine CD133 expression in clinical samples of primary tumors, as well as liver and peritoneal metastases. Under hypoxia, the expression levels of hypoxia-inducible factor (HIF)-1α and the epithelial-mesenchymal transition markers N-cadherin and vimentin were significant

SUBMITTER: Okada M 

PROVIDER: S-EPMC7681219 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature