Astrocytic TSPO Upregulation Appears Before Microglial TSPO in Alzheimer's Disease.

ABSTRACT:

Background

In vivo PET/SPECT imaging of neuroinflammation is primarily based on the estimation of the 18 kDa-translocator-protein (TSPO). However, TSPO is expressed by different cell types which complicates the interpretation.

Objective

The present study evaluates the cellular origin of TSPO alterations in Alzheimer's disease (AD).

Methods

The TSPO cell origin was evaluated by combining radioactive imaging approaches using the TSPO radiotracer [125I]CLINDE and fluorescence-activated cell sorting, in a rat model of AD (TgF344-AD) and in AD subjects.

Results

In the hippocampus of TgF344-AD rats, TSPO overexpression not only concerns glial cells but the increase is visible at 12 and 24 months in astrocytes and only at 24 months in microglia. In the temporal cortex

SUBMITTER: Tournier BB 

PROVIDER: S-EPMC7683091 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature