Data on using single- and mixed-mode resins for capture chromatography of recombinant human thioredoxin from Escherichia coli.

ABSTRACT: This paper provides the data collected from screening chromatographic resins for their ability to bind and purify recombinant human thioredoxin from Escherichia coli lysate. This data was used by "Capture chromatography with mixed-mode resins: A case study with recombinant human thioredoxin from Escherichia coli" [1] to determine the optimal resin to use as a capture step to initiate downstream processing of thioredoxin. Five chromatography resins were screened using a 96-well filter plate to experiment on a wide range of pH and conductivity conditions in a shorter amount of time while saving on materials. Thioredoxin-producing E. coli was cultivated, harvested, and lysed according to Ravi et al [1]. Thioredoxin containing lysate was dialyzed into the binding conditions, pH f

SUBMITTER: Ravi A 

PROVIDER: S-EPMC7683221 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature