Regulation of Gluconeogenesis by Aldo-keto-reductase 1a1b in Zebrafish.

ABSTRACT: Regulation of glucose homeostasis is a fundamental process to maintain blood glucose at a physiological level, and its dysregulation is associated with the development of several metabolic diseases. Here, we report on a zebrafish mutant for Aldo-keto-reductase 1a1b (akr1a1b) as a regulator of gluconeogenesis. Adult akr1a1b ^-/- mutant zebrafish developed fasting hypoglycemia, which was caused by inhibiting phosphoenolpyruvate carboxykinase (PEPCK) expression as rate-limiting enzyme of gluconeogenesis. Subsequently, glucogenic amino acid glutamate as substrate for gluconeogenesis accumulated in the kidneys, but not in livers, and induced structural and functional pronephros alterations in 48-hpf akr1a1b ^-/- embryos. Akr1a1b

SUBMITTER: Li X 

PROVIDER: S-EPMC7683270 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature