Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids.

ABSTRACT: Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organization and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, incl

SUBMITTER: Smits LM 

PROVIDER: S-EPMC7683480 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature