Repetitive Fed-Batch: A Promising Process Mode for Biomanufacturing With E. coli.

ABSTRACT: Recombinant protein production with Escherichia coli is usually carried out in fed-batch mode in industry. As set-up and cleaning of equipment are time- and cost-intensive, it would be economically and environmentally favorable to reduce the number of these procedures. Switching from fed-batch to continuous biomanufacturing with microbials is not yet applied as these cultivations still suffer from time-dependent variations in productivity. Repetitive fed-batch process technology facilitates critical equipment usage, reduces the environmental fingerprint and potentially increases the overall space-time yield. Surprisingly, studies on repetitive fed-batch processes for recombinant protein production can be found for yeasts only. Knowledge on repetitive fed-batch cultivation technology

SUBMITTER: Kopp J 

PROVIDER: S-EPMC7683717 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature