Turnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes.

ABSTRACT: Virus-induced plant diseases in cultivated plants cause important damages in yield. Although the mechanisms of virus infection are intensely studied at the cell biology level, only little is known about the molecular dialog between the invading virus and the host genome. Here we describe a combinatorial genome-wide approach to identify networks of sRNAs-guided post-transcriptional regulation within local Turnip mosaic virus (TuMV) infection sites in Brassica napus leaves. We show that the induction of host-encoded, virus-activated small interfering RNAs (vasiRNAs) observed in virus-infected tissues is accompanied by site-specific cleavage events on both viral and host RNAs that recalls the activity of small RNA-induced silencing complexes (RISC). Cleavage events also involve virus-derived

SUBMITTER: Pitzalis N 

PROVIDER: S-EPMC7683744 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature