Isolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14.

ABSTRACT:

Background

Wheat sheath blight, a soil borne fungal disease caused by Rhizoctonia cerealis, is considered as one of the most serious threats to wheat worldwide. Bacillus subtilis Z-14 was isolated from soil sampled from a wheat rhizosphere and was confirmed to have strong antifungal activity against R. cerealis.

Results

An antifungal protein, termed F2, was isolated from the culture supernatant of Z-14 strain using precipitation with ammonium sulfate, anion exchange chromatography, and reverse phase chromatography. Purified F2 had a molecular mass of approximately 8 kDa, as assessed using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Edman degradation was used to determine the amino acid sequence of the N-terminus, which was NH₂ASGGTVGIYGANMRS. This s

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7684727 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature