Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58.

ABSTRACT: A unique feature of Hodgkin lymphoma (HL) is the presence of CD4+ T cells that surround, protect, and promote survival of tumor cells. The adhesion molecules involved in this so-called T-cell rosetting are important components of the immunological synapse (IS). However, it is unknown whether this synapse is fully assembled and leads to T-cell activation by enabling interaction between the T-cell receptor (TCR) and human leukocyte antigen class II (HLA-II). We established a novel rosetting model by coculturing HLA-II-matched peripheral blood mononuclear cells with HL cell lines and showed IS formation with activation of rosetting T cells. HLA-II downregulation by class II transactivator knockout did not affect the extent of rosetting, but almost completely abrogated T-cell activation. Intri

SUBMITTER: Veldman J 

PROVIDER: S-EPMC7685209 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature