Expression and Characterization of Manduca sexta Stress Responsive Peptide-1; an Inducer of Antimicrobial Peptide Synthesis.

ABSTRACT: In response to stress conditions such as wounding or infections in insects, several short peptides are processed to act as cytokines that induce AMP gene expression. To study their structure-activity relationship, immune inducibility, tissue specificity, stress responsiveness, and development relatedness, we chemically synthesized Manduca sexta stress response peptide-1, a 25-residue peptide with one disulfide bond (SRP1: FGVRVGTCPSGYVRRGTFCFPDDDY). Upon injection of the SRP1 into naïve larvae, several antimicrobial peptide genes were expressed at higher levels. The mRNA levels of SRP1 increased significantly in hemocytes and fat body after larvae were challenged with a mixture of bacteria and β-1,3-glucan. The expression patterns of SRP1 and its target genes are somewhat different

SUBMITTER: Schrag LG 

PROVIDER: S-EPMC7685292 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature