SiCTeC: An inexpensive, easily assembled Peltier device for rapid temperature shifting during single-cell imaging.

ABSTRACT: Single-cell imaging, combined with recent advances in image analysis and microfluidic technologies, have enabled fundamental discoveries of cellular responses to chemical perturbations that are often obscured by traditional liquid-culture experiments. Temperature is an environmental variable well known to impact growth and to elicit specific stress responses at extreme values; it is often used as a genetic tool to interrogate essential genes. However, the dynamic effects of temperature shifts have remained mostly unstudied at the single-cell level, due largely to engineering challenges related to sample stability, heatsink considerations, and temperature measurement and feedback. Additionally, the few commercially available temperature-control platforms are costly. Here, we report an inexp

SUBMITTER: Knapp BD 

PROVIDER: S-EPMC7685484 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature