Cellular sensing platform with enhanced sensitivity based on optogenetic modulation of cell homeostasis.

ABSTRACT: We demonstrate a new biosensing concept with impact on the development of rapid, point of need cell based sensing with boosted sensitivity and wide relevance for bioanalysis. It involves optogenetic stimulation of cells stably transfected to express light sensitive protein channels for optical control of membrane potential and of ion homeostasis. Time-lapse impedance measurements are used to reveal cell dynamics changes encompassing cellular responses to bioactive stimuli and optically induced homeostasis disturbances. We prove that light driven perturbations of cell membrane potential induce homeostatic reactions and modulate transduction mechanisms that amplify cellular response to bioactive compounds. This allows cell based biosensors to respond more rapidly and sensitively to low conce

SUBMITTER: Gheorghiu M 

PROVIDER: S-EPMC7685521 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature